From: Subject: The Scientist :: Fruit bats may be Ebola reservoir, Dec. 1, 2005 Date: Sat, 10 Dec 2005 13:31:42 +0200 MIME-Version: 1.0 Content-Type: multipart/related; boundary="----=_NextPart_000_0000_01C5FD8E.0EA75D80"; type="text/html" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1441 This is a multi-part message in MIME format. ------=_NextPart_000_0000_01C5FD8E.0EA75D80 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.the-scientist.com/news/20051201/01 The Scientist :: Fruit bats may be Ebola reservoir, = Dec. 1, 2005

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Fruit bats may be Ebola reservoir

Researchers find antibody and RNA evidence that three = fruit bat=20 species harbor Ebola virus

By Melissa Lee Phillips

Three species of fruit bats may be the = long-sought reservoirs of Ebola virus in Africa, = according to=20 a report = in this=20 week's Nature. Researchers have found antibodies = specific to=20 Ebola as well as viral RNA sequences in symptom-less bats, = which=20 they collected in central Africa during Ebola outbreaks in = humans=20 and other great apes.

"It's a very significant finding for all of = us who=20 have been working with this virus for a very long time," = said Joseph McCormick of the University of = Texas School=20 of Public Health at Brownsville, who was not involved in the = study.

From 2001 to 2003, Eric Leroy of the = International=20 Center for Medical Research in Gabon and his colleagues = collected=20 more than 1,000 small vertebrates in areas of Gabon and the = Republic=20 of the Congo that were stricken with primate outbreaks of = Ebola=20 hemorrhagic fever.

Of these animals, the researchers detected=20 immunoglobulin G (IgG) specific for Ebola virus in 16 fruit bats from three = species.=20 They also used nested PCR to fish for Ebola genetic = material, and=20 found Ebola RNA sequences in the liver and spleen of 13 = other bats=20 from the same three species, but not in any other = animals.

Sequencing the viral RNA revealed seven = different=20 fragments, all of which cluster in the Ebola Zaire clade =96 = one of=20 four known subtypes of Ebola virus. The fragments differed = between=20 and within bat species. This diversity suggests that Ebola = virus has=20 been present in bats for some time, Leroy told The = Scientist=20 in an Email, indicating they are probably its primary = reservoir.

However, Leroy and his colleagues did not = isolate the=20 complete virus from any of the bat samples, which would have = made=20 their case stronger, Leroy said. "I'm not too surprised at = that,"=20 said Clarence J. Peters of the University of = Texas=20 Medical Branch in Galveston, who was not involved in the = research.=20 The need to use nested PCR to extract RNA suggests that the = bats=20 carried a very low viral load, Peters said.

Ebola RNA and Ebola-specific IgG were never = detected=20 in the same individual bat, which was a bit of a surprise, = the=20 authors say. It's possible that bats recently infected with = RNA may=20 not have yet developed an immune response and that bats positive = for IgG may=20 have cleared the virus from their systems, according to = Leroy. This=20 result suggests that Ebola infection in bats may not be = chronic, but=20 it's too soon to know for sure, McCormick said.

The bats positive for antibodies or RNA = were not only=20 of different species but different genera, which is "a = little=20 unusual" for a viral reservoir, Peters told The = Scientist.=20 Chronic viral infections in rodents, for example, are = usually=20 species-specific, he said. "It makes you wonder if maybe = these guys=20 are all three getting it from somewhere else." Many viruses = are=20 transmitted between vertebrates by arthropods, but "nobody = really=20 thinks that arthopods are involved in maintaining Ebola," = Peters=20 said.

A different animal transmitting the virus = between bat=20 species "seems somewhat unlikely," according to McCormick. = Future=20 studies on the genetic diversity of Ebola viruses in bats = and the=20 geographic range of infected bats will likely help to answer = the=20 question, he said. If Ebola viruses are "fairly diverse = coming out=20 of bats from different regions," that strengthens the case = for bats=20 as the source.

Leroy's work gives "the best leading = candidate ever"=20 for an Ebola reservoir, Peters said, but to be sure that = these bats=20 are the primary reservoir, "you'd have to work out all the = details=20 of the natural cycle of the virus," including whether Ebola=20 infection in bats is chronic, becomes latent and is later=20 reactivated, or is completely wiped out by the immune = system.

Links for this = article=20

Charles Choi, "Bats = might be=20 origin of SARS," The Scientist, September 30, 2005. = http://www.the-scientist.com/news/20050930/01=20

E.M. Leroy et al., = "Fruit bats as=20 reservoirs of Ebola virus," Nature, December 1, 2005. = http://www.nature.com/

Joseph McCormick http://www.sph.uth.tmc.edu/cid/research/McCormick.htm= =20

D. Secko, "In a raft = with Ebola=20 VP40," The Scientist, December 2, 2003. http://www.the-scientist.com/news/20031202/01=20

Clarence J. Peters http://www.utmb.edu/pathology/administration/faculty/deta= ils.asp?who=3Dcjpeters=20

J. Perkel, "Human = Ebola vaccine=20 trial begins," The Scientist, November 24, 2003. http://www.the-scientist.com/news/20031124/06=20

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=A9 2005 The Scientist, unless = otherwise stated |=20 E-mail: support@the-scientist.com

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